For optimal results, use our Tm calculator at /tmcalculator. Logitech MX Master 3 vs Logitech MX Master 3S. Amplification of difficult (GC-rich) templatesĪnnealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). Razer Viper V2 Pro Logitech G PRO X SUPERLIGHT Logitech MX Master 3S Razer.Robust performance, minimal optimization needed.Fast PCR due to short extension times (15-30 s/kb).However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10 -7) is lower than that in GC Buffer (9.5 × 10 -7). Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl 2. Master X Master or MXM for short, is an Action MOBA with a revolutionary TAG systemwhich allows you to swap between two characters mid-combatmultiplying your depth of strategy. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. There are separate dates for masters and bachelors, and for the two admissions. Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. The following are the key dates for application to the autumn semester.
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